Part:BBa_K1471009:Design
RBS - PhyB - AGS - VP16 - NLS - PolyA.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 507
Illegal BamHI site found at 589
Illegal XhoI site found at 540
Illegal XhoI site found at 559 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 756
Design Notes
This part was designed to produce the complete protein complex for PhyB comformations and the red lgiht switch system.
Source
Arabidopsis thaliana genome
References
Taiz,L. and E.Zeiger.(2006) Fisiologia Vegetal.Universitat Jaume. 3rd Edition.Pages 7-27 Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/ Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/ Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*. (n.d.). Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α. Retrieved October 11, 2014, from http://www.jbc.org/content/282/8/5101.full Nuclear localization sequence. (2014, November 10). Wikipedia. Retrieved October 11, 2014, from http://en.wikipedia.org/wiki/Nuclear_localization_sequence Result Filters. (n.d.). National Center for Biotechnology Information. Retrieved October 11, 2014, from http://www.ncbi.nlm.nih.gov/pubmed/8241603 Poly(A)-tail profiling reveals an embryonic switch in translational control. (n.d.). Nature journal. Retrieved October 11, 2014, from http://www.nature.com/nature/journal/v508/n7494/full/nature13007.html The Poly(A) Tail. (n.d.). The RNA Underworld. Retrieved October 12, 2014, from http://aghunt.wordpress.com/2008/06/05/the-polya-tail/ poly-A tail. (n.d.). Nature.com. Retrieved October 12, 2014, from http://www.nature.com/scitable/definition/poly-a-tail-276
Tiley LS1, Madore SJ, Malim MH, Cullen BR.(1992).The VP16 Transcription Activation Domain Is Functional When Targeted to a Promoter-proximal RNA Sequence. Genes Development. Volume 6; Issue 11; Pages: 2077-2087.
Carbone, A-L, M. Moroni, P-J Groot-Kormelink, and I. Bermudez(2009).Pentameric concatenated (α4)2(β2)3 and (α4)3(β2)2 nicotinic acetylcholine receptors: subunit arrangement determines functional expression. Britain Journal of Pharmacology.Volue 156; Issue 6; Pages 970–981.